p53 d0 1  (Cell Signaling Technology Inc)

Journal: Translational Cancer Research

Article Title: Silencing PPA1 promotes the survival of non-small cell lung cancer A549 cells under glucose-starved conditions

doi: 10.21037/tcr-2025-1106

Figure Lengend Snippet: Silencing PPA1 enhances A549 survival under glucose-free condition. (A) CCK-8 detection of cell viability of the A549-shCtrl, A549-shPPA1-1, and A549-shPPA1-2 cell lines under glucose starvation. (B) Colony formation of A549-shCtrl, A549-shPPA1-1, and A549-shPPA1-2 cell lines under glucose-starved conditions (crystal violet staining; magnification, ×1). (C) Cell cycle analysis by flow cytometry in A549-shCtrl, A549-shPPA1-1, and A549-shPPA1-2 cells under glucose starvation. (D) Western blotting detection of the expression of proliferative proteins in PPA1-silenced A549 cells under glucose starvation. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001, compared with the shCtrl group; N=3. shPPA1-1 vs. shCtrl: p21-0h, P=0.004; CDK2-0h, P<0.001; Ki-67-0h, P<0.001; p53-0h, P=0.001; p21-24h, P=0.03; CDK2-24h, P=0.008; Ki-67-24h, P=0.003; p53-24h, P<0.001; shPPA1-2 vs. shCtrl: p21-0h, P<0.001; CDK2-0h, P<0.001; Ki-67-0h, P<0.001; p53-0h, P=0.03; p21-24h, P<0.001; CDK2-24h, P=0.007; Ki-67-24h, P=0.01; p53-24h, P=0.001. shCtrl group: empty vector control group; shPPA1-1 and shPPA1-2 groups: PPA1-silencing groups. PE-A, phycoerythrin-area; PPA1, inorganic pyrophosphatase 1.

Article Snippet: PPA1 antibody (B-8), beta-actin antibody (C4), and TP53 antibody (D0-1) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), Ki-67 antibody was purchased from Abcam (Cambridge, UK).

Techniques: CCK-8 Assay, Staining, Cell Cycle Assay, Flow Cytometry, Western Blot, Expressing, Plasmid Preparation, Control

Journal: Cell reports

Article Title: Protein adduction causes non-mutational inhibition of p53 tumor suppressor

doi: 10.1016/j.celrep.2023.112024

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse anti-p53 (D0-1) , Millipore , Cat# OP43.

Techniques: Virus, Recombinant, Staining, Reverse Transcription, Fractionation, Luciferase, Reporter Assay, Sequencing, ChIP-qPCR, Software

Journal: BMC Cancer

Article Title: Integral analysis of p53 and its value as prognostic factor in sporadic colon cancer

doi: 10.1186/1471-2407-13-277

Figure Lengend Snippet: Patients’ characteristics

Article Snippet: Heat induced antigen retrieval (HIAR) was performed as described elsewhere [ ] and staining was carried out with the mouse anti-human monoclonal antibodies directed against p53 (clone D0-7, 1:1000 dilution) (Lab Vision NeoMarkers, Fremont, CA, USA). p53 was scored in four different categories based on any level of nuclear staining, like previously described [ ] by an experienced pathologist (HM) and a pathology resident (AFS): completely negative; 1- 25% positive nuclei (indicative of a wild type state); 25-75% positive nuclei and >75% positive nuclei.

Techniques: Clone Assay

Journal: BMC Cancer

Article Title: Integral analysis of p53 and its value as prognostic factor in sporadic colon cancer

doi: 10.1186/1471-2407-13-277

Figure Lengend Snippet: Results of a) SNP array on reference chromosome and chr.17p b) FISH on Chr. 17 (the green signal corresponds to the centromere probe and the red signal to the p53 probe).

Article Snippet: Heat induced antigen retrieval (HIAR) was performed as described elsewhere [ ] and staining was carried out with the mouse anti-human monoclonal antibodies directed against p53 (clone D0-7, 1:1000 dilution) (Lab Vision NeoMarkers, Fremont, CA, USA). p53 was scored in four different categories based on any level of nuclear staining, like previously described [ ] by an experienced pathologist (HM) and a pathology resident (AFS): completely negative; 1- 25% positive nuclei (indicative of a wild type state); 25-75% positive nuclei and >75% positive nuclei.

Techniques:

Journal: BMC Cancer

Article Title: Integral analysis of p53 and its value as prognostic factor in sporadic colon cancer

doi: 10.1186/1471-2407-13-277

Figure Lengend Snippet: Associations between clinicopathological variables and p53 functionality

Article Snippet: Heat induced antigen retrieval (HIAR) was performed as described elsewhere [ ] and staining was carried out with the mouse anti-human monoclonal antibodies directed against p53 (clone D0-7, 1:1000 dilution) (Lab Vision NeoMarkers, Fremont, CA, USA). p53 was scored in four different categories based on any level of nuclear staining, like previously described [ ] by an experienced pathologist (HM) and a pathology resident (AFS): completely negative; 1- 25% positive nuclei (indicative of a wild type state); 25-75% positive nuclei and >75% positive nuclei.

Techniques: Functional Assay, Amplification, Clone Assay

Journal: BMC Cancer

Article Title: Integral analysis of p53 and its value as prognostic factor in sporadic colon cancer

doi: 10.1186/1471-2407-13-277

Figure Lengend Snippet: List of genes differentially expressed between functional p53 and non functional p53 groups

Article Snippet: Heat induced antigen retrieval (HIAR) was performed as described elsewhere [ ] and staining was carried out with the mouse anti-human monoclonal antibodies directed against p53 (clone D0-7, 1:1000 dilution) (Lab Vision NeoMarkers, Fremont, CA, USA). p53 was scored in four different categories based on any level of nuclear staining, like previously described [ ] by an experienced pathologist (HM) and a pathology resident (AFS): completely negative; 1- 25% positive nuclei (indicative of a wild type state); 25-75% positive nuclei and >75% positive nuclei.

Techniques: Functional Assay, Significance Assay, Expressing, Binding Assay

Journal: BMC Cancer

Article Title: Integral analysis of p53 and its value as prognostic factor in sporadic colon cancer

doi: 10.1186/1471-2407-13-277

Figure Lengend Snippet: Kaplan Meier plots for CSS according to p53 functionality.

Article Snippet: Heat induced antigen retrieval (HIAR) was performed as described elsewhere [ ] and staining was carried out with the mouse anti-human monoclonal antibodies directed against p53 (clone D0-7, 1:1000 dilution) (Lab Vision NeoMarkers, Fremont, CA, USA). p53 was scored in four different categories based on any level of nuclear staining, like previously described [ ] by an experienced pathologist (HM) and a pathology resident (AFS): completely negative; 1- 25% positive nuclei (indicative of a wild type state); 25-75% positive nuclei and >75% positive nuclei.

Techniques:

Journal: BMC Cancer

Article Title: Integral analysis of p53 and its value as prognostic factor in sporadic colon cancer

doi: 10.1186/1471-2407-13-277

Figure Lengend Snippet: Kaplan Meier plots for CSS according to CSNK1A1 expression stratified on the base of p53 functionality.

Article Snippet: Heat induced antigen retrieval (HIAR) was performed as described elsewhere [ ] and staining was carried out with the mouse anti-human monoclonal antibodies directed against p53 (clone D0-7, 1:1000 dilution) (Lab Vision NeoMarkers, Fremont, CA, USA). p53 was scored in four different categories based on any level of nuclear staining, like previously described [ ] by an experienced pathologist (HM) and a pathology resident (AFS): completely negative; 1- 25% positive nuclei (indicative of a wild type state); 25-75% positive nuclei and >75% positive nuclei.

Techniques: Expressing

Journal: BMC Cancer

Article Title: Integral analysis of p53 and its value as prognostic factor in sporadic colon cancer

doi: 10.1186/1471-2407-13-277

Figure Lengend Snippet: Kaplan Meier for CSS according to p53 and CSNK1A1 combination variable.

Article Snippet: Heat induced antigen retrieval (HIAR) was performed as described elsewhere [ ] and staining was carried out with the mouse anti-human monoclonal antibodies directed against p53 (clone D0-7, 1:1000 dilution) (Lab Vision NeoMarkers, Fremont, CA, USA). p53 was scored in four different categories based on any level of nuclear staining, like previously described [ ] by an experienced pathologist (HM) and a pathology resident (AFS): completely negative; 1- 25% positive nuclei (indicative of a wild type state); 25-75% positive nuclei and >75% positive nuclei.

Techniques:

Journal: BMC Cancer

Article Title: Integral analysis of p53 and its value as prognostic factor in sporadic colon cancer

doi: 10.1186/1471-2407-13-277

Figure Lengend Snippet: Cox Proportional Hazards Model: multivariate survival analysis

Article Snippet: Heat induced antigen retrieval (HIAR) was performed as described elsewhere [ ] and staining was carried out with the mouse anti-human monoclonal antibodies directed against p53 (clone D0-7, 1:1000 dilution) (Lab Vision NeoMarkers, Fremont, CA, USA). p53 was scored in four different categories based on any level of nuclear staining, like previously described [ ] by an experienced pathologist (HM) and a pathology resident (AFS): completely negative; 1- 25% positive nuclei (indicative of a wild type state); 25-75% positive nuclei and >75% positive nuclei.

Techniques:

Journal: NPJ Breast Cancer

Article Title: Retinoblastoma protein expression and its predictors in triple-negative breast cancer

doi: 10.1038/s41523-020-0160-4

Figure Lengend Snippet: Proportions of negative, low positive, and positive p53 by molecular features at presentation.

Article Snippet: Similarly, p53 staining was performed on TMA sections using a mouse monoclonal antibody to human p53 (clone D0–1, ImmunoTech, Hostivař, Czech Republic) and scored as negative (<1% nuclear staining), low positive (≥1% to <10% nuclear staining), or high positive (≥10% nuclear staining).

Techniques:

Journal: NPJ Breast Cancer

Article Title: Retinoblastoma protein expression and its predictors in triple-negative breast cancer

doi: 10.1038/s41523-020-0160-4

Figure Lengend Snippet: Molecular features at presentation by retinoblastoma protein status among the full cohort ( n = 180).

Article Snippet: Similarly, p53 staining was performed on TMA sections using a mouse monoclonal antibody to human p53 (clone D0–1, ImmunoTech, Hostivař, Czech Republic) and scored as negative (<1% nuclear staining), low positive (≥1% to <10% nuclear staining), or high positive (≥10% nuclear staining).

Techniques: Staining

Journal: Cancer research

Article Title: NORE1A Tumor Suppressor Candidate Modulates p21 CIP1 via p53

doi: 10.1158/0008-5472.CAN-08-3672

Figure Lengend Snippet: A, loss of NORE1A expression correlates with loss of p21CIP1 expression and inversely with p53 mutation in human HCC. HCC samples were examined for NORE1A promoter hypermethylation (HYP) and LOH, as well as for NORE1A and p21CIP1 expression and p53 mutation. Tumors 1 to 35 are HCCP (survival, <3 y). Tumors 36 to 60 are HCCB (survival, >3 y). Protein lysates were immunoblotted with NORE1A and p21CIP1 antibodies. In each case, Western blot analysis revealed that NORE1A methylation corresponded with reduced NORE1A expression compared with normal liver and, in every case but one, reduced p21CIP1 expression. NORE1A inactivation and p53 mutation were mutually exclusive. B, representative Western blot analysis is shown. Actin serves as a loading control. HCCB, HCC with better prognosis; HCCP, HCC with poorer prognosis; NL, normal livers. HuH2 human HCC (C) and A549 NSCLC (D) cell lines were treated with 5-Aza-C and analyzed by Western blot for the expression of NORE1A and p21CIP1. Actin serves as a loading control.

Article Snippet: The following antibodies were used: anti-p21 CIP1 (Novus Biologicals); mouse monoclonal anti–cyclin A, anti–cyclin E, rabbit polyclonal anti-CDK2, p53-D0–1 (Santa Cruz Biotechnology); rabbit polyclonal anti-NORE1A ( 5 ); HA and FLAG antibodies (Sigma).

Techniques: Expressing, Mutagenesis, Western Blot, Methylation, Control

Journal: Cancer research

Article Title: NORE1A Tumor Suppressor Candidate Modulates p21 CIP1 via p53

doi: 10.1158/0008-5472.CAN-08-3672

Figure Lengend Snippet: NORE1A activates p21CIP1 protein expression via p53. A, HuH6 (p53 wild type; H6) and HuH7 (p53 mutant; H7) human HCC cell lines were transfected with pCDNAFLAG NORE1A, and the expression of NORE1A and p21CIP1 protein was determined by Western blot. NORE1A induced p21CIP1 only in the p53 wild-type cell line, whereas the total levels of p53 did not change in both cell lines. B, Hek-293 cells were transfected with NORE1A in the presence or absence of p53siRNA or a p53 dominant-negative construct. An HA-tagged p53 expression vector serves as a positive control. siRNA to p53 or the p53 dominant-negative inhibited NORE1A induced p21CIP1 expression. C, NORE1A induces nuclear translocation of wild-type p53. HuH6 (p53 wild type; H6) and HuH7 (p53 mutant; H7) HCC cell lines were transiently transfected with pCDNAFLAG NORE1A and fractionated into cytoplasmic (i) and nuclear (ii) fractions before Western blotting for p53. NORE1A had no effect on p53 levels in HuH7 cells, but it promoted progressive nuclear accumulation of p53 in HuH6 cells.

Article Snippet: The following antibodies were used: anti-p21 CIP1 (Novus Biologicals); mouse monoclonal anti–cyclin A, anti–cyclin E, rabbit polyclonal anti-CDK2, p53-D0–1 (Santa Cruz Biotechnology); rabbit polyclonal anti-NORE1A ( 5 ); HA and FLAG antibodies (Sigma).

Techniques: Expressing, Mutagenesis, Transfection, Western Blot, Dominant Negative Mutation, Construct, Plasmid Preparation, Positive Control, Translocation Assay